PCRmax® Eco 48 Real-Time qPCR System

Learn more about the PCRmax® Eco 48 qPCR System

The Eco 48 qPCR system workflow is based on three simple steps:

  • Load samples into the 48-well plate using the backlit Eco Dock and place the plate into the Eco 48.
  • Run experiment.
  • The Eco 48 software provides instrument control, data collection, and data analysis. The most common qPCR parameters are automatically in place. These can be easily changed on the computer to meet specific experimental requirements.

Features and benefits

  • MIQE compliant open license software giving confidence when it comes to publishing your data.
  • HRM functionality as standard: with the ability to discriminate class IV SNP >99% of the time.
  • Eco 48 allows for simple multiplex analysis with up to four dyes at once
  • World most thermally accurate block gives consistent data every time.
  • High uniformity provides high quality data. Fast cycling enables several experiments per day, all at an economical price.
  • Fastest block-based real-time PCR system. 40 cycles in 40 minutes standardly and 40 cycles in as little as 15 minutes after optimization of temperatures and hold times.
  • Eco 48 is an open platform that can utilize any chemistry, dye, or PCR reagent.
  • Easy to use software, making analysis simple and fast.
  • Industry-leading data quality.
  • Eco 48 format provides a small 34.5cm x 31cm (13.6” × 12.2”) footprint to save bench space

Eco 48 qPCR system thermal technology

Eco 48 qPCR system thermal technology

qPCR specificity and efficiency relies on precise temperature control during the denaturation and annealing steps. For the highest accuracy, temperature must remain uniform across the entire heat block, ensuring that all samples are processed equally. The patented hollow silver and gold liquid filled thermal block design of Eco 48 achieves this with a unique heating and cooling system that provides accurate ±0.1ºC (measured at 95 ºC) temperature control and quickly cycles from one temperature to the next not requiring any settle time at all to hit your specified temperatures.

Eco 48 qPCR system thermal optical system

Eco 48 qPCR Thermal Optical System

The Eco 48 real-time PCR system contains an advanced high-performance optical system that delivers precise and sensitive fluorescence detection, facilitating all 4 color multiplex applications. The system is provided factory-calibrated for use with SYBR®, FAM™, HEX®, VIC®, ROX®, Cy®5 and can also be used with any qPCR fluorophores overlapping with these calibrated dyes. For excitation, two panels of 48 fixed LEDs provide excitation energy of distinct spectra, enabling broad range excitation. Each of the 48 LEDs illuminates a specific plate well location, eliminating the optical distortion created by most stationary optical systems.

Rapid cycling

Eco 48 has rapid heating and cooling rates. Average ramp rates of 5.5°C/sec to aid in reduction of overall PCR run times. The typical run time for a 40 cycle PCR protocol is around 40 minutes. In testing, the quickest optimized program enabled a 40-cycle qPCR experiment to complete in just under 20 minutes without sacrificing thermal uniformity in the process as the block still achieves a thermal uniformity of ±0.1ºC (measured at 95 ºC) at these speeds.

HRM analysis protocols are supported by continuous data acquisition during the melt for increased data collection and reduced run times. The user can change the plate setup and perform data analysis after the run is complete.

High throughput

Eco 48 thermal control is superior to traditional 96-well peltier systems where thermal efficiency decreases as distance from the peltier increases. With the Eco 48 qPCR system, faster and more precise cycling increases sample throughput because the need for multiple replicates is reduced by increased uniformity across the block.

The Eco 48 qPCR system’s well-to-well, plate-to-plate and instrument-to-instrument results are as consistent as running a single experiment. To take advantage of this, Eco 48 has a function in the software where different experiments are reliably combined and analyzed as one. High uniformity reduces the need for experimental replicates which saves on precious samples, eliminates the need for more expensive instruments, reduces reagent running costs and ensures you can do more with the sample you have.

Software

The Eco 48 qPCR system come with two distinct software, the first, Eco Control, drives the units set up and experimental runs while the second, Eco Study is the analysis package. Both are open licenses—they can be can installed on an unlimited amount of PCs, and MIQE compliant.

Eco 48 Software
  • Eco 48 qPCR system software uses a unique icon-driven user interface to simplify experimental design and setup.
  • Pre-set thermal profile defaults are provided for commonly used experimental protocols.
  • Temperature and time for each protocol step can easily be changed by click-and-drag action.
  • Experiment templates can be customized and saved for future use.
  • With the Eco 48 qPCR system and software, data collection is monitored in real time, allowing researchers to access run viability immediately.
  • The user-friendly data analysis interface also allows researchers to easily view the amplification plot, melt curve analysis, and the analyzed results, including Cq values, PCR efficiency, R2 and Y-intercept.
  • Data can be exported into Excel or CSV and custom reports generated directly into PowerPoint or PDF formats.
  • High-resolution images can be directly exported in multiple image formats, ready to use in any presentation.
  • Conforms to Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, making data analysis and submission for publication review more efficient.

Eco Control software

  • User-installable, intuitive, easy to use software that integrates user control, real-time data collection, and advanced data analysis.
  • Supplied fully featured including HRM capability.
  • New software enables a widened chemistry library and contains new thermal profiles.

Eco Study software

  • Data analysis and multi-experiment analysis.
  • Allows higher throughput, pool separate plates together and analyze as one experiment.
  • Almost limitless sample volume.
  • Supplied on a USB stick