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Separate protein groups on agarose beads with tris(2-aminoethyl)amine (TREN), iminodiacetic acid (IDA), or nitrilotriacetic acid (NTA). Select from uncharged TREN, or charged or uncharged IDA and NTA. Charged media contains IDA or NDA with Ni, Co, Cu, or Zn ions, which are immobilized on the media via the chelating ligand. Metal ion capacity is 50 to 60 µmol/mL.
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